Editorial Type:
Article Category: Research Article
 | 
Online Publication Date: 04 Jun 2025

miR-135a promotes tumor invasion by targeting BACH1 and GATA3 in gastric cancer

MD,
MD,
MD,
MD, and
MD PhD
DOI: 10.9738/INTSURG-D-24-00006
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Abstract

AIM

The aim of this study was to investigate the effect of miR-135a/BACH1/GATA3 signaling on the proliferation, apoptosis, invasion, and migration of gastric cancer cells.

METHODS

Sixty specimens of cancer tissues and sixty specimens of corresponding adjacent tissues in gastric cancer patients were collected. qPCR and immunohistochemistry were used to detect the expression levels of miR-135a, BACH1 and GATA3 in cancer tissues and adjacent tissues. miR-135a inhibitor, miR-141 mimic, BACH1 inhibitor and GATA3 inhibitor carriers were established. Cell proliferation was detected by CCK8, the invasion ability of cells in vitro was evaluated by MTT, and the cell apoptosis of each group was detected by flow cytometry.

RESULTS

The results of RT-PCR showed that the expression levels of miR-135a in gastric cancer tissues were significantly higher than those in adjacent tissues, while the expression levels of BACH1 and GATA3 in gastric tissues were significantly lower than those in adjacent tissues (P<0.05). Interfering with miR-135a increased both BACH1 and GATA3 expression levels; overexpression of miR-135a significantly reduced BACH1 and GATA3 expression levels. Interference with BACH1 and GATA3 significantly enhanced the proliferative capacity of gastric cancer cells. Overexpression of miR-135a significantly increased apoptosis, whereas interference with BACH1 and GATA3 increased apoptosis.

CONCLUSION

miR-135a participates in the development of gastric cancer by regulating gastric cancer cell proliferation and apoptosis by targeting BACH1 and GATA3.

Contributor Notes

Corresponding author: Yanfei Zhu, MD, Department of General Surgery, Wuxi, People's Hospital of Nanjing Medical University, No. 299, Qingyang Road, Wuxi, 214023, Jiangsu Province, China. zhuyanfei_2002@163.com Telephone: +86-510-85350091 Fax: +86-510-82828435
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